Application | Comment | Organism |
---|---|---|
analysis | the highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances the specificity of simplex and multiplex PCR assays | Pyrococcus woesei |
diagnostics | the RadA protein proves to be useful in allelic discrimination assay of HADHA gene associated with long-chain 3-hydroxylacyl-CoA dehydrogenase deficiency that in infancy may lead to hypotonia, serious heart and liver problems and even sudden death | Pyrococcus woesei |
Cloned (Comment) | Organism |
---|---|
gene radA DNA and amino acid sequence determination and analysis, recombinant overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Pyrococcus woesei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pyrococcus woesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Pyrococcus woesei | - |
ADP + phosphate | - |
? | |
ATP + H2O | Pyrococcus woesei DSM 3773 | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus woesei | A0A0D3MA54 | gene radA | - |
Pyrococcus woesei DSM 3773 | A0A0D3MA54 | gene radA | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) using ammonium sulfate fractionation and nickel affinity chromatography, to homogeneity | Pyrococcus woesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Pyrococcus woesei | ADP + phosphate | - |
? | |
ATP + H2O | - |
Pyrococcus woesei DSM 3773 | ADP + phosphate | - |
? | |
additional information | enzyme RadA binds both to ssDNA and dsDNA. The highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances the specificity of simplex and multiplex PCR assays | Pyrococcus woesei | ? | - |
? | |
additional information | enzyme RadA binds both to ssDNA and dsDNA. The highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances the specificity of simplex and multiplex PCR assays | Pyrococcus woesei DSM 3773 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
undecamer | - |
Pyrococcus woesei |
Synonyms | Comment | Organism |
---|---|---|
RadA | - |
Pyrococcus woesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Pyrococcus woesei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
94 | - |
purified recombinant His-tagged RadA, remains almost unaffected after 4 h incubation | Pyrococcus woesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
ATPase assay at | Pyrococcus woesei |
8 | - |
DNA binding assay at | Pyrococcus woesei |